Effective Chikungunya virus-like particle vaccine produced in insect cells
Autor(es): Metz Stefan W, Gardner Joy, Geertsema Corinne, Le Thuy T, Goh Lucas, Vlak Just M, Suhrbier Andreas, Pijlman Gorben P
Resumo: The emerging arthritogenic, mosquito-borne chikungunya virus (CHIKV) causes severe disease in humans - represents a serious public health threat in countries where Aedes spp mosquitoes are present. This study describes for the first time the successful production of CHIKV virus-like particles (VLPs) in insect cells using recombinant baculoviruses. This well-established expression system is rapidly scalable to volumes required for epidemic responses - proved well suited for processing of CHIKV glycoproteins - production of enveloped VLPs. Herein we show that a single immunization with 1 µg of non-adjuvanted CHIKV VLPs induced high titer neutralizing antibody responses - provided complete protection against viraemia - joint inflammation upon challenge with the Réunion Isl- CHIKV strain in an adult wild-type mouse model of CHIKV disease. CHIKV VLPs produced in insect cells using recombinant baculoviruses thus represents as a new, safe, non-replicating - effective vaccine c-idate against CHIKV infections.
Imprenta: PLoS Neglected Tropical Diseases, v. 7, n. 3, p. e2124, 2013
Identificador do objeto digital: 10.1371/journal.pntd.0002124
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Inflammation ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Vaccine ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Epidemic ; Chikungunya virus - Immunology ; Chikungunya virus - Public health
Data de publicação: 2013
