Dried-blood spots: A cost-effective field method for the detection of Chikungunya virus circulation in remote areas
Autor(es): Andriam-imby Soa Fy, Heraud Jean-Michel, R-rianasolo Laurence, Rafis-ratantsoa Jean Théophile, Andriamamonjy Seta, Richard Vincent
Resumo: In 2005, there were outbreaks of febrile polyarthritis due to Chikungunya virus (CHIKV) in the Comoros Isl-s. CHIKV then spread to other isl-s in the Indian Ocean: La Réunion, Mauritius, Seychelles - Madagascar. These outbreaks revealed the lack of surveillance - preparedness of Madagascar - other countries. Thus, it was decided in 2007 to establish a syndrome-based surveillance network to monitor dengue-like illness. This study aims to evaluate the use of capillary blood samples blotted on filter papers for molecular diagnosis of CHIKV infection. Venous blood samples can be difficult to obtain - the shipment of serum in appropriate temperature conditions is too costly for most developing countries. Venous blood - dried-blood blotted on filter paper (DBFP) were collected during the last CHIKV outbreak in Madagascar (2010) - as part of our routine surveillance of dengue-like illness. All samples were tested by real-time RT-PCR - results with serum - DBFP samples were compared for each patient. The sensitivity - specificity of tests performed with DBFP, relative to those with venous samples (defined as 100%) were 93.1% (95% CI:[84.7-97.7]) - 94.4% (95% CI:[88.3-97.7]), respectively. The Kappa coefficient 0.87 (95% CI:[0.80-0.94]) was excellent. This study shows that DBFP specimens can be used as a cost-effective alternative sampling method for the surveillance - monitoring of CHIKV circulation - emergence in developing countries, - probably also for other arboviruses. The loss of sensitivity is insignificant - involved a very small number of patients, all with low viral loads. Whether viruses can be isolated from dried blood spots remains to be determined.
Imprenta: PLoS Neglected Tropical Diseases, v. 7, n. 7, p. e2339, 2013
Identificador do objeto digital: 10.1371/journal.pntd.0002339
Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Viral infections ; Chikungunya virus - Molecular methods ; Chikungunya virus - PCR detection ; Chikungunya virus - Real Time PCR ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Molecular screening ; Chikungunya virus - Dengue ; Chikungunya virus - Public health
Data de publicação: 2013
