DNA vaccine initiates replication of live attenuated Chikungunya virus in vitro and elicits protective immune response in mice
Autor(es): Tretyakova Irina, Hearn Jason, Wang Eryu, Weaver Scott, Pushko Peter
Resumo: Chikungunya virus (CHIKV) causes outbreaks of chikungunya fever worldwide - represents an emerging p-emic threat. Vaccine development against CHIKV has proved challenging. Currently there is no approved vaccine or specific therapy for the disease. To develop novel experimental CHIKV vaccine, we used novel immunization DNA (iDNA) infectious clone technology, which combines the advantages of DNA - live attenuated vaccines. Here we describe an iDNA vaccine composed of plasmid DNA that encode the full-length infectious genome of live attenuated CHIKV clone 181/25 downstream from a eukaryotic promoter. The iDNA approach was designed to initiate replication of live vaccine virus from the plasmid in vitro - in vivo. Experimental CHIKV iDNA vaccines were prepared - evaluated in cultured cells - in mice. Transfection with 10 ng of iDNA was sufficient to initiate replication of vaccine virus in vitro. Vaccination of BALB/c mice with a single 10 ?g of CHIKV iDNA plasmid resulted in seroconversion, elicitation of neutralizing antibodies, - protection from experimental challenge with a neurovirulent CHIKV. Live attenuated CHIKV 181/25 vaccine can be delivered in vitro - in vivo by using DNA vaccination. The iDNA approach appears to represent a promising vaccination strategy for CHIK - other alphaviral diseases.
Palavras-Chave: CHIKV; Chikungunya fever; Chikungunya virus; DNA vaccine; Alphavirus; Live attenuated vaccine
Imprenta: The Journal of Infectious Diseases, v. 209, n. 12, p. 1882-1890, 2014
Identificador do objeto digital: 10.1093/infdis/jiu114
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Cytopathology ; Chikungunya virus - DNA ; Chikungunya virus - Genome ; Chikungunya virus - Immune response ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Immune response ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya virus - Molecular methods ; Chikungunya Virus - Virus ; Chikungunya virus - Vaccine ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Immunology
Data de publicação: 2014
