Differential proteome analysis of Chikungunya virus-infected new-born mice tissues reveal implication of stress, inflammatory and apoptotic pathways in disease pathogenesis
Autor(es): Dhanwani Rekha, Khan Mohsin, Alam Syed Imtiyaz, Rao Putcha Venkata Lakshmana, Parida Manmohan
Resumo: Chikungunya infection is a major disease of public health concern. The recurrent outbreaks of this viral disease - its progressive evolution dem-s a potential strategy to underst- major aspects of its pathogenesis. Unlike other alphaviruses, Chikungunya virus (CHIKV) pathogenesis is poorly understood. In every consecutive outbreak, some new symptoms associated with virulence - disease manifestations are being reported such as neurological implication, increased severity - enhanced vector competence. In order to unravel the mechanism of the disease process, proteomic analysis was performed to evaluate the host response in CHIKV-infected mice tissues. Comparative analysis of the multiple gels representing the particular tissue extract from mock - CHIKV-infected tissues revealed a drastic reprogramming of physiological conditions through 35 - 15 differentially expressed proteins belonging to different classes such as stress, inflammation, apoptosis, urea cycle, energy metabolism, etc. from liver - brain, respectively. Based on the alterations obtained in the CHIKV mouse model, most of the aspects of CHIKV infection such as disease severity, neurological complications, disease susceptibility - immunocompetence could be defined. This is the first report unravelling the complicated pathways involved in the mechanism of Chikungunya disease pathogenesis employing proteomic approach.
Imprenta: Proteomics, v. 11, n. 10, p. 1936-1951, 2011
Identificador do objeto digital: 10.1002/pmic.201000500
Descritores: Chikungunya virus - Biochemistry ; Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Cytopathology ; Chikungunya virus - Molecular structure ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Proteome ; Chikungunya virus - Inflammation ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Public health
Data de publicação: 2011
