Autor(es): Thio Christina Li-Ping, Yusof Rohana, Abdul-Rahman Puteri Shafinaz Akmar, Karsani Saiful Anuar

Resumo: Chikungunya virus (CHIKV) is an emerging mosquito-borne alphavirus that has caused multiple unprecedented - re-emerging outbreaks in both tropical - temperate countries. Despite ongoing research efforts, the underlying factors involved in facilitating CHIKV replication during early infection remains ill-characterized. The present study serves to identify host proteins modulated in response to early CHIKV infection using a proteomics approach. The whole cell proteome profiles of CHIKV-infected - mock control WRL-68 cells were compared - analyzed using two-dimensional gel electrophoresis (2-DGE). Fifty-three spots were found to be differentially modulated - 50 were successfully identified by MALDI-TOF/TOF. Eight were significantly up-regulated - 42 were down-regulated. The mRNA expressions of 15 genes were also found to correlate with the corresponding protein expression. STRING network analysis identified several biological processes to be affected, including mRNA processing, translation, energy production - cellular metabolism, ubiquitin-proteasome pathway (UPP) - cell cycle regulation. This study constitutes a first attempt to investigate alteration of the host cellular proteome during early CHIKV infection. Our proteomics data showed that during early infection, CHIKV affected the expression of proteins that are involved in mRNA processing, host metabolic machinery, UPP, - cyclin-dependent kinase 1 (CDK1) regulation (in favour of virus survival, replication - transmission). While results from this study complement the proteomics results obtained from previous late host response studies, functional characterization of these proteins is warranted to reinforce our underst-ing of their roles during early CHIKV infection in humans.

Imprenta: PloS One, v. 8, n. 4, p. e61444, 2013

Identificador do objeto digital: 10.1371/journal.pone.0061444

Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Proteome ; Chikungunya virus - RNA ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Chikungunya fever

Data de publicação: 2013