Autor(es): Tchankouo-Nguetcheu Stéphane, Khun Huot, Pincet Laurence, Roux Pascal, Bahut Muriel, Huerre Michel, Guette Catherine, Choumet Valérie

Resumo: Arthropod borne virus infections cause several emerging - resurgent infectious diseases. Among the diseases caused by arboviruses, dengue - chikungunya are responsible for a high rate of severe human diseases worldwide. The midgut of mosquitoes is the first barrier for pathogen transmission - is a target organ where arboviruses must replicate prior to infecting other organs. A proteomic approach was undertaken to characterize the key virus/vector interactions - host protein modifications that happen in the midgut for viral transmission to eventually take place. Using a proteomics differential approach with two-Dimensional Differential in-Gel Electrophoresis (2D-DIGE), we defined the protein modulations in the midgut of Aedes aegypti that were triggered seven days after an oral infection (7 DPI) with dengue 2 (DENV-2) - chikungunya (CHIKV) viruses. Gel profile comparisons showed that the level of 18 proteins was modulated by DENV-2 only - 12 proteins were modulated by CHIKV only. Twenty proteins were regulated by both viruses in either similar or different ways. Both viruses caused an increase of proteins involved in the generation of reactive oxygen species, energy production, - carbohydrate - lipid metabolism. Midgut infection by DENV-2 - CHIKV triggered an antioxidant response. CHIKV infection produced an increase of proteins involved in detoxification. Our study constitutes the first analysis of the protein response of Aedes aegypti's midgut infected with viruses belonging to different families. It shows that the differentially regulated proteins in response to viral infection include structural, redox, regulatory proteins, - enzymes for several metabolic pathways. Some of these proteins like antioxidant are probably involved in cell protection. On the other h-, we propose that the modulation of other proteins like transferrin, hsp60 - alpha glucosidase, may favour virus survival, replication - transmission, suggesting a subversion of the insect cell metabolism by the arboviruses.

Imprenta: PloS One, v. 5, n. 10, 2010

Identificador do objeto digital: 10.1371/journal.pone.0013149

Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Dengue

Data de publicação: 2010

INDICADORES

ACESSO