Autor(es): Thio Christina Li-Ping, Yusof Rohana, Ashrafzadeh Ali, Bahari Syareena, Abdul-Rahman Puteri Shafinaz, Karsani Saiful Anuar

Resumo: The Chikungunya virus (CHIKV) is an arthropod borne virus. In the last 50 years, it has been the cause of numerous outbreaks in tropical - temperate regions, worldwide. There is limited underst-ing regarding the underlying molecular mechanisms involved in CHIKV replication - how the virus interacts with its host. In the present study, comparative proteomics was used to identify secreted host proteins that changed in abundance in response to early CHIKV infection. Two-dimensional gel electrophoresis was used to analyse - compare the secretome profiles of WRL-68 cells infected with CHIKV against mock control WRL-68 cells. The analysis identified 25 regulated proteins in CHIKV infected cells. STRING network analysis was then used to predict biological processes that may be affected by these proteins. The processes predicted to be affected include signal transduction, cellular component - extracellular matrix (ECM) organization, regulation of cytokine stimulus - immune response. These results provide an initial view of CHIKV may affect the secretome of infected cells during early infection. The results presented here will compliment earlier results from the study of late host response. However, functional characterization will be necessary to further enhance our underst-ing of the roles played by these proteins in the early stages of CHIKV infection in humans.

Imprenta: PloS One, v. 10, n. 6, p. e0129033, 2015

Identificador do objeto digital: 10.1371/journal.pone.0129033

Descritores: Chikungunya virus - Cell ; Chikungunya virus - Cytopathology ; Chikungunya virus - Immune response ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Proteome ; Chikungunya virus - Immune response ; Chikungunya Virus - Virus ; Chikungunya virus - Molecular screening

Data de publicação: 2015

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