Diagnosis of Dengue infection using conventional and biosensor based techniques
Autor(es): Parkash Om, Shueb Rafidah Hanim
Resumo: Dengue is an arthropod-borne viral disease caused by four antigenically different serotypes of dengue virus. This disease is considered as a major public health concern around the world. Currently, there is no licensed vaccine or antiviral drug available for the prevention - treatment of dengue disease. Moreover, clinical features of dengue are indistinguishable from other infectious diseases such as malaria, chikungunya, rickettsia - leptospira. Therefore, prompt - accurate laboratory diagnostic test is urgently required for disease confirmation - patient triage. The traditional diagnostic techniques for the dengue virus are viral detection in cell culture, serological testing, - RNA amplification using reverse transcriptase PCR. This paper discusses the conventional laboratory methods used for the diagnosis of dengue during the acute - convalescent phase - highlights the advantages - limitations of these routine laboratory tests. Subsequently, the biosensor based assays developed using various transducers for the detection of dengue are also reviewed.
Palavras-Chave: NS1; Biosensor; Dengue; Dengue specific IgM; Detection; Diagnosis; rt-PCR; Viral isolation
Imprenta: Viruses, v. 7, n. 10, p. 5410-5427, 2015
Identificador do objeto digital: 10.3390/v7102877
Descritores: Chikungunya virus - Cell ; Chikungunya virus - RNA ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya virus - PCR detection ; Chikungunya virus - RT-PCR ; Chikungunya virus - Serological diagnosis ; Chikungunya Virus - Virus ; Chikungunya virus - Vaccine ; Chikungunya virus - Dengue ; Chikungunya virus - Public health
Data de publicação: 2015
