Autor(es): Cheng Ming Soon, Ho Jia Shin, Tan Cheong Huat, Wong Jeslyn Pei Sze, Ng Lee Ching, Toh Chee-Seng

Resumo: A sensitive membrane-based electrochemical nanobiosensor is developed for the detection of dengue type 2 virus (DENV-2) using nanoporous alumina-modified platinum electrode. Its sensing mechanism relies on the monitoring of electrode's Faradaic current response toward redox probe, ferrocenemethanol, which is sensitive toward the formation of immune complexes within the alumina nanochannels. Anti-DENV-2 monoclonal antibody (clone 3H5, isotype IgG) is used as the biorecognition element in this work. The stepwise additions of antibody, bovine serum albumin (BSA) - DENV-2 are characterized by differential pulse voltammetry (DPV). A low detection limit of 1 pfu mL(-1) with linear range from 1 to 10(3) pfu mL(-1) (R(2)=0.98) can be achieved by the nanobiosensor. The nanobiosensor is selective toward DENV-2 with insignificant cross reaction with non-specific viruses, Chikungunya virus, West Nile virus - dengue type 3 virus (DENV-3). Relative st-ard deviation (RSD) for triplicate analysis of 5.9% indicates an acceptable level of reproducibility. The first direct quantitation of DENV-2 concentration in whole mosquito vector is demonstrated using this electrochemical nanobiosensor.

Palavras-Chave: Dengue; Virus; Electroanalytical chemistry; Nanoporous membrane; Biomedical; Diagnostics

Imprenta: Analytica Chimica Acta, v. 725, p. 74-80, 2012

Identificador do objeto digital: 10.1016/j.aca.2012.03.017

Descritores: Chikungunya virus - Flaviviridae ; Chikungunya virus - Immune response ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Immune response ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Dengue ; Chikungunya virus - Immunology ; Chikungunya virus - Public health

Data de publicação: 2012

INDICADORES

ACESSO