Development of an attenuated strain of Chikungunya virus for use in vaccine production
Autor(es): Levitt N H, Ramsburg H H, Hasty S E, Repik P M, Cole F E, Lupton H W
Resumo: An attenuated chikungunya (CHIK) virus clone was developed for production of a live vaccine for human use. CHIK strain 15561 was subjected to 18 plaque-to-plaque passages in MRC-5 cultures before CHIK 181/clone 25 was selected as vaccine seed based on homogeneous small plaque size, suckling mouse avirulence, reduced monkey viraemia - genetic stability. Oligonucleotide mapping demonstrated differences between parent - clone. Vaccine (pilot-lot production) elicited neutralizing antibody - protected mice - rhesus monkeys against challenge. After challenge, viraemias were absent in vaccinated monkeys. Vaccine was then produced - tested in accordance with governmental regulatory requirements of human use.
Palavras-Chave: Chikungunya virus; Clone selection; Vaccine production; Vaccine testing
Imprenta: Vaccine, v. 4, n. 3, p. 157-162, 1986
Identificador do objeto digital: 10.1016/0264-410X(86)90003-4
Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya virus - Antibodies ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Vaccine ; Chikungunya virus - Immunology
Data de publicação: 1986
