Autor(es): Kishishita Natsuko, Takeda Naokazu, Anuegoonpipat Atchareeya, Anantapreecha Surapee

Resumo: Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that causes chikungunya fever in Africa, South Asia, - Southeast Asia. Because the mosquito vector Aedes albopictus is present in habitats across Europe, North America, - East Asia, CHIKV has become a serious worldwide public health concern. Infection with CHIKV typically causes fever, rash, myalgia, - arthralgia. One of the important questions yet to be answered is how the host immune system is involved in the development of this disease. In this study, we prepared a CHIKV-pseudotyped lentiviral vector for use in a safe - convenient neutralization (NT) assay - analyzed its efficacy. The CHIKV-pseudotyped lentiviral vector was prepared by cotransfection with plasmids encoding the CHIKV glycoproteins E3, E2, 6k, - E1, packaging elements, - a luciferase reporter. This alternative to native CHIKV can be safely h-led in a biosafety level 2 facility. The NT assay was optimized using sera from CHIKV-immunized mice - then applied to human patient sera. The majority of the serum samples from patients with chikungunya in Thail- showed robust neutralization activities, with titers that were tightly correlated with those determined by a conventional NT assay. Moreover, there was a strong correlation with the CHIKV antibody titers as determined by enzyme-linked immunosorbent assay. Thus, the CHIKV-pseudotyped-lentiviral-vector-based NT assay system is a powerful tool for examining the neutralization activity of patient sera, which will lead to a better underst-ing of the immune responses involved in CHIKV infection.

Imprenta: Journal of Clinical Microbiology, v. 51, n. 5, p. 1389-1395, 2013

Identificador do objeto digital: 10.1128/JCM.03109-12

Descritores: Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Serology ; Chikungunya virus - Viral infections ; Chikungunya virus - Serology ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Immunology ; Chikungunya virus - Public health

Data de publicação: 2013

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