Autor(es): Cecilia D, Kakade M, Alagarasu K, Patil J, Salunke A, Parashar D, Shah P S

Resumo: Dengue - chikungunya viruses co-circulate - cause infections that start with similar symptoms but progress to radically different outcomes. Therefore, an early diagnostic test that can differentiate between the two is needed. A single-step multiplex real-time RT-PCR assay was developed that can simultaneously detect - quantitate RNA of all dengue virus (DENV) serotypes - chikungunya virus (CHIKV). The sensitivity was 100 % for DENV - 95.8 % for CHIKV, whilst the specificity was 100 % for both viruses when compared with conventional RT-PCR. The detection limit ranged from 1 to 50 plaque-forming units. The assay was successfully used for differential diagnosis of dengue - chikungunya in Pune, where the viruses co-circulate.

Palavras-Chave: Dengue; Chikungunya; Multiplex real time RT-PCR assay; Diagnosis

Imprenta: Archives of Virology, v. 160, n. 1, p. 323-327, 2015

Identificador do objeto digital: 10.1007/s00705-014-2217-x

Descritores: Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya virus - RNA ; Chikungunya virus - Viral infections ; Chikungunya virus - PCR detection ; Chikungunya virus - Real Time PCR ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Epidemiology ; Chikungunya virus - Public health

Data de publicação: 2015

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