Autor(es): Xiao-Ping Kang, Yong-Qiang Li, Qing-Ge Sun, Hong Liu, Qing-Yu Zhu, Yin-Hui Yang

Resumo: Some highly pathogenic viruses, such as Chikungunya virus, Japanese encephalitis virus, Yellow fever virus, Dengue virus, Hanta virus, SARS-CoV, - H5N1 avian influenza virus can cause severe infectious diseases. However, the consensus method for detecting these viruses has not been well established. A rapid - sensitive microarray approach for detection of these viruses - a panel of specific probes covering nine genera - 16 virus species were designed. 70-mer oligonucleotides were used at the genus level - 50-mer oligonucleotides were at the species level, respectively. To decrease the interference of the host genome in hybridization, the consensus genus primers were designed - used to reverse transcribe only virus genome. The synthesis of the second str- was carried out with a r-om primer sequence (5'-GTTTCCCAGTAGGTCTCNNNNNNNN-3'). The amplified products were labeled - processed for microarray analyses. This microarray-based method used the highly conserved consensus primers to synthesize specifically the virus cDNA - could identify effectively Chikungunya virus, Japanese encephalitis virus, Yellow fever virus, Dengue virus, Tick borne encephalitis virus, - H5N1 avian influenza virus. Using this method, one unknown virus isolated from pig brain in Shanxi Province, China was identified. This method may have an important potential application for the diagnosis of virus infection.

Imprenta: Journal of Medical Virology, v. 81, n. 11, p. 1945-1950; 2009

Identificador do objeto digital: 10.1002/jmv.21602

Descritores: Chikungunya virus - Flaviviridae ; Chikungunya virus - Genome ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Epidemiology

Data de publicação: 2009

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