Determinants of vector specificity of o'nyong nyong and chikungunya viruses in Anopheles and Aedes mosquitoes
Autor(es): Vanlandingham Dana L, Tsetsarkin Konstantin, Klingler Kimberly A, Hong Chao, McElroy Kate L, Lehane Michael J, Higgs Stephen
Resumo: The alphaviruses o'nyong nyong virus (ONNV) - chikungunya virus (CHIKV) provide a unique system to study the viral genes involved in vector specificity. ONNV infects both anopheline - culicine mosquitoes, whereas CHIKV infects only culicine mosquitoes. In this study, chimeric viruses were constructed that contained genes from both ONNV - CHIKV. These chimeras - previously described full-length infectious clones of ONNV - CHIKV were evaluated in Anopheles gambiae - Aedes aegypti mosquitoes. Virus derived from the infectious clones of ONNV - CHIKV retained the vector specificity of the parental viruses. All six of the chimeras were found to infect Ae. aegypti mosquitoes at high rates but only the chimera containing viral genes encoding all of the structural proteins of ONNV was able to infect An. gambiae mosquitoes. These data indicate that all of the viral structural proteins are necessary for ONNV to infect An. gambiae mosquitoes.
Imprenta: The American Journal of Tropical Medicine and Hygiene, v. 74, n. 4, p. 663-669, 2006
Descritores: Chikungunya virus - Cytopathology ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya Virus - Virus ; Chikungunya virus - Public health
Data de publicação: 2006
