Detection of viral antigen, IgM and IgG antibodies in cerebrospinal fluid of Chikungunya patients with neurological complications
Autor(es): Kashyap Rajpal S, Morey Shweta H, Chandak Nitin H, Purohit Hemant J, Taori Girdhar M, Daginawala Hatim F
Resumo: During Chikungunya virus (CHIKV) epidemic in Nagpur, India, we identified some suspected Chikungunya patients with neurological complications. Early - cost-effective diagnosis of these patients remains problematic despite many new advanced diagnostic methods. A reliable diagnostic test, which could be performed in any st-ard pathology laboratory, would help to obtain definitive early diagnosis of CHIKV patients with neurological complications. In our laboratory, in-house ELISA protocol for viral antigen, immunoglobulin M (IgM) - IgG detection has been developed - assessed for the diagnosis of CHIKV patients with neurological complications. Cerebrospinal fluid samples of forty-six patients who developed neurological symptoms within two months of CHIKV infections along with control subjects were included in the study - were analyzed for the presence of antigens - of IgM - IgG using an ELISA protocol. The ELISA method for antigen detection yielded 80% sensitivity - 87% specificity for the diagnosis of CHIKV patients with neurological complications. The sensitivity for detection of IgM 48% or IgG 63% was significantly lower than the antigen assay (80%). The detection of viral antigen in CSF of CHIKV patients with neurological complications by ELISA method gave a more reliable diagnosis than antibodies detection that can be used to develop an immunodiagnostic assay with increased sensitivity - specificity.
Imprenta: Cerebrospinal Fluid Research, v. 7, p. 12, 2010
Identificador do objeto digital: 10.1186/1743-8454-7-12
Descritores: Chikungunya virus - Biochemistry ; Chikungunya virus - Cytopathology ; Chikungunya virus - Antibodies ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Epidemic
Data de publicação: 2010
