Autor(es): Wong Hui Vern, Vythilingam Indra, Sulaiman Wan Yusof Wan, Lulla Aleksei, Merits Andres, Chan Yoke Fun, Sam I-Ching

Resumo: Vertical transmission may contribute to the maintenance of arthropod-borne viruses, but its existence in chikungunya virus (CHIKV) is unclear. Experimental vertical transmission of infectious clones of CHIKV in Aedes aegypti mosquitoes from Malaysia was investigated. Eggs - adult progeny from the second gonotrophic cycles of infected parental mosquitoes were tested. Using polymerase chain reaction (PCR), 56.3% of pooled eggs - 10% of adult progeny had detectable CHIKV RNA, but no samples had detectable infectious virus by plaque assay. Transfected CHIKV RNA from PCR-positive eggs did not yield infectious virus in BHK-21 cells. Thus, vertical transmission of viable CHIKV was not demonstrated. Noninfectious CHIKV RNA persists in eggs - progeny of infected Ae. aegypti, but the mechanism - significance are unknown. There is insufficient evidence to conclude that vertical transmission exists in CHIKV, as positive results reported in previous studies were almost exclusively based only on viral RNA detection.

Imprenta: The American Journal of Tropical Medicine and Hygiene, v. 94, n. 1, p. 182-186, 2016

Identificador do objeto digital: 10.4269/ajtmh.15-0318

Descritores: Chikungunya virus - Cell ; Chikungunya virus - RNA ; Chikungunya virus - PCR detection ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission

Data de publicação: 2016

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