Detection of Chikungunya virus in Nepal
Autor(es): P-ey Basu Dev, Neupane Biswas, Pandey Kishor, Tun Mya Myat Ngwe, Morita Kouichi
Resumo: Chikungunya virus (CHIKV) is an emerging alphaviral disease - a public health problem in South Asia including Nepal in recent years. In this study, sera were collected from patients presenting with fever, headache, muscular pain, fatigue, - joint pain of both upper - lower extremities. A total of 169 serum samples were tested for CHIKV - dengue virus (DENV) by using Immunoglobulin M (IgM) - Immunoglobulin G (IgG) antibody using enzyme-linked immunosorbent assay (ELISA) method during August to November 2013. Results showed that 3.6% - 27.8% samples were positive for CHIKV - DENV IgM positive, respectively. Similarly, results of IgG showed 3.0% samples were positive for CHIKV IgG - 29.0% were for DENV IgG positive. Further, a 50% focal reduction neutralization test (FRNT50) was performed to confirm the presence of CHIKV, which demonstrated that 8.9% of CHIKV IgM -/or IgG ELISA positive possessed neutralizing anti-CHIK antibodies. To our knowledge, this is the first report in which the presence of CHIKV is confirmed in Nepalese patients by FRNT50. Basic scientists - clinicians need to consider CHIKV as a differential diagnosis in febrile Nepalese patients, - policy makers should consider appropriate surveillance - actions for control strategies.
Imprenta: The American Journal of Tropical Medicine and Hygiene, v. 93, n. 4, p. 697-700, 2015
Identificador do objeto digital: 10.4269/ajtmh.15-0092
Descritores: Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Epidemiology ; Chikungunya virus - Immunology ; Chikungunya virus - Public health
Data de publicação: 2015
