Copy number variation of Chikungunya ECSA virus with disease symptoms among Indian patients
Autor(es): Dutta Sudip Kumar, Pal Tithi, Saha Bibhuti, Mandal Syamsundar, Tripathi Anusri
Resumo: After a gap of three decades, from 2005 onwards, a series of Chikungunya virus (CHIKV) outbreaks occurred worldwide. This study was performed to detect CHIKV infection, its genotype among symptomatic Eastern Indian patients - to analyze any association between the presence of CHIKV genome in patient body with appearance of disease symptoms (n = 199). Plasma-extracted viral RNA was reverse transcribed to cDNA - PCR-amplified followed by agarose gel electrophoresis. Viral load among CHIKV-positive patients was determined by real time RT-PCR. CHIKV-IgM in sera was detected by ELISA. Sequencing - phylogenetic analysis of plasma-extracted PCR products was done. CHIKV genome - IgM were detected among 65.3% (n = 130) - 41.2% (n = 82) patients respectively. Joint swelling was significantly associated with CHIKV infection (P-value: 0.0003). CHIKV PCR positive patients were grouped in two categories: Group-I: viral load <10(4) copies/ml - Group-II: viral load ?10(4) copies/ml. Higher number of acute stage patients clustered in Group-II. Fever - joint swelling were significantly more prevalent among Group-II patients, whereas rash - diarrhoea among Group-I patients (P-value <0.05). Patient-isolated CHIKV sequences clustered with CHIKV ECSA genotypes in the phylogenetic tree, with two types of CHIKV strains found to circulate among them-as indicated by their different nucleotide sequences. This is the first study detecting the presence of CHIKV ECSA genotype among Eastern Indian patients. Fever - joint swelling might have appeared first followed by rash, diarrhea during disease progression-as indicated by CHIK viral load in patients. Thus, viral load can be used as unique diagnostic - prognostic marker of Chikungunya disease pathogenesis.
Palavras-Chave: Chikungunya viral load; IgM ELISA; RT-PCR; Fever; Joint swelling; Myalgia
Imprenta: Journal of Medical Virology, v. 86, n. 8, p. 1386-1392, 2014
Identificador do objeto digital: 10.1002/jmv.23794
Descritores: Chikungunya virus - Biochemistry ; Chikungunya virus - Cytopathology ; Chikungunya virus - DNA ; Chikungunya virus - Genome ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya virus - Antibodies ; Chikungunya virus - Viral infections ; Chikungunya virus - Real Time PCR ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Molecular screening ; Chikungunya virus - Chikungunya fever
Data de publicação: 2014
