Connective tissue metabolism in chikungunya patients
Autor(es): Lokireddy Sudarsanareddy, Vemula Sarojamma, Vadde Ramakrishna
Resumo: Chikungunya (CHIK) fever is a viral disease transmitted to humans by the bite of Chikungunya virus (CHIK virus) infected Aedes mosquitoes. CHIK virus is a member of the Alphavirus genus of the family Togaviridae. Previous reports have indicated that infection with CHIK virus produces an acute arthritis in human hosts by large area of necrosis - collagenosis or fibrosis. We carried out the present study to determine the effect of chikungunya on the collagen - connective tissue metabolism in 75 chikungunya-affected people. First, we screened for mucopolysaccharides in urine by Cetyl Trimethyl Ammonium Bromide (CTAB) test. Appearance of heavy precipitate indicates the presence of higher levels of mucopolysaccharides - later quantified by DMB dye method. The urinary mucopolysaccharide in CHIK patients was 342 +/- 45 mg/l compared to healthy controls (45 +/- 5.6 mg/l). The collagen building blocks, proline - hydroxyproline were also measured in CHIK patients - observed higher excretion compared to healthy controls. Urinary excretions hydroxyproline was greater than the proline levels. These results indicate that CHIK virus infection affects - damage the cartilage - connective metabolism - releases the degraded products from the tissue - responsible for increasing the levels of proline, hydroxyproline - mucopolysaccharides in CHIK affected patients.
Imprenta: Virology Journal, v. 5, p. 31, 2008
Identificador do objeto digital: 10.1186/1743-422X-5-31
Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever
Data de publicação: 2008
