Autor(es): Potiwat Rutcharin, Komalamisra Narumon, Thavara Usavadee, Tawatsin Apiwat, Siriyasatien Padet

Resumo: Aedes albopictus C6/36 cell line was used to evaluate dengue virus serotype-3 (DENV-3) - chikungunya virus (CHIKV) co-infection. Virus infection was determined using a one-step duplex reverse transcriptase polymerase chain reaction (D- RT-PCR). D-RT-PCR was positive for both viruses when equal multiplicity of infection (MOI) was utilized. Co-infection with different titers between a higher CHIKV titer (MOI = 1.0) than DENV-3 titer (MOI = 0.1) showed similar results with that of equal titer. However, co-infection with a lower CHIKV titer (MOI = 0.1) than DENV-3 titer (MOI = 1.0) revealed positive results for only dengue virus infection, suggesting DENV competitive suppression of CHIKV. This competitive suppression occurred in mixed-infection samples but not in individually double infection (super infection) samples which produced both dengue - chikungunya virus progenies. Both virus replications depend on the amount of virus titer rather than serial infection. These findings have provided information regarding pathogen-pathogen interaction in host cell, which could be used to predict outbreaks, - to develop virus detection - vector control.

Palavras-Chave: Chikungunya virus; Dengue virus; Aedes albopictus C6/36 cell line; Competitive suppression

Imprenta: The Southeast Asian Journal of Tropical Medicine and Public Health, v. 42, n. 6, p. 1388-1894, 2011

Descritores: Chikungunya virus - Cell ; Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Viral infections ; Chikungunya virus - PCR detection ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Public health

Data de publicação: 2011

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