Comparison of PanBio dengue duo enzyme-linked immunosorbent assay (ELISA) and MRL dengue fever virus immunoglobulin M capture ELISA for diagnosis of dengue virus infections in Southeast Asia
Autor(es): Cuzzubbo A J, Vaughn D W, Nisalak A, Solomon T, Kalayanarooj S, Aaskov J, Dung N M, Devine P L
Resumo: The performances of the MRL dengue fever virus immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (ELISA) - the PanBio Dengue Duo IgM capture - IgG capture ELISA were compared. Eighty sera from patients with dengue virus infections, 24 sera from patients with Japanese encephalitis (JE), - 78 sera from patients with nonflavivirus infections, such as malaria, typhoid, leptospirosis, - scrub typhus, were used. The MRL test showed superior sensitivity for dengue virus infections (94 versus 89%), while the PanBio test showed superior specificity for JE (79 versus 25%) - other infections (100 versus 91%). The PanBio ELISA showed better overall performance, as assessed by the sum of sensitivity - specificity (F value). When dengue virus - nonflavivirus infections were compared, F values of 189 - 185 were obtained for the PanBio - MRL tests, respectively, while when dengue virus infections - JE were compared, F values of 168 - 119 were obtained. The results obtained with individual sera in the PanBio - MRL IgM ELISAs showed good correlation, but this analysis revealed that the cutoff value of the MRL test was set well below that of the PanBio test. Comparing the sensitivity - specificity of the tests at different cutoff values (receiver-operator analysis) revealed that the MRL - PanBio IgM ELISAs performed similarly in distinguishing dengue virus from nonflavivirus infections, although the PanBio IgM ELISA showed significantly better distinction between dengue virus infections - JE. The implications of these findings for the laboratory diagnosis of dengue are discussed.
Imprenta: Clinical and Diagnostic Laboratory Immunology, v. 6, n. 5, p. 705-712, 1999
Descritores: Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Immunology ; Chikungunya virus - Public health
Data de publicação: 1999
