Autor(es): Santhosh S R, Dash P K, Parida M M, Khan M, Tiwari M, Lakshmana Rao P V

Resumo: The resurgence of Chikungunya virus (CHIKV) in the form of unprecedented explosive epidemic after a gap of 32 years in India is a point of major public health concern. In 2007 again there was outbreak in Kerala, India, affecting more than 25,000 cases with many reported mortalities. To underst- the molecular basis of this high virulence - its implication in large-scale epidemic, a detailed systematic serological, virological - molecular investigation was undertaken with the epidemic samples of Kerala-2007. The comparative analysis of full genome sequence of Chikungunya virus isolate of 2007 with 2006 revealed three unique substitutions in structural - non-structural genes of 2007 isolate [two in E1 region (V14A - A226V) - one in Nsp1 (M184T)]. Our finding further substantiates the association of A226V shift in E1 gene with evolutionary success possibly due to adaptation in the mosquito vector with progression of epidemic, as observed in Reunion Isl-. This A226V shift which was absent in all 2006 Indian isolates, is found to be present in the four 2007 isolates, analysed in this study. These unique molecular features of the 2007 isolates with the progression of the epidemic from 2005 to 2007 demonstrate their high evolutionary - epidemic potential - thereby suggesting possible implication in higher magnitude - virulence of this outbreak.

Palavras-Chave: Chikungunya virus; Outbreak; Molecular evolution; Phylogeny; Sequence

Imprenta: Virus Research, v. 135, n. 1, p. 36-41, 2008

Identificador do objeto digital: 10.1016/j.virusres.2008.02.004

Descritores: Chikungunya virus - DNA ; Chikungunya virus - Genome ; Chikungunya virus - Molecular structure ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya virus - Antibodies ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Epidemic ; Chikungunya virus - Epidemiology ; Chikungunya virus - Immunology ; Chikungunya virus - Public health

Data de publicação: 2008

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