Comparative analysis of viral RNA signatures on different RIG-I-like receptors
Autor(es): Sanchez David Raul Y, Combredet Chantal, Sismeiro Odile, Dillies Marie-Agnès, Jagla Bernd, Coppée Jean-Yves, Mura Marie, Guerbois Galla Mathilde, Despres Philippe, Tangy Frédéric, Komarova Anastassia V
Resumo: The RIG-I-like receptors (RLRs) play a major role in sensing RNA virus infection to initiate - modulate antiviral immunity. They interact with particular viral RNAs, most of them being still unknown. To decipher the viral RNA signature on RLRs during viral infection, we tagged RLRs (RIG-I, MDA5, LGP2) - applied tagged protein affinity purification followed by next-generation sequencing (NGS) of associated RNA molecules. Two viruses with negative- - positive-sense RNA genome were used: measles (MV) - chikungunya (CHIKV). NGS analysis revealed that distinct regions of MV genome were specifically recognized by distinct RLRs: RIG-I recognized defective interfering genomes, whereas MDA5 - LGP2 specifically bound MV nucleoprotein-coding region. During CHIKV infection, RIG-I associated specifically to the 3' untranslated region of viral genome. This study provides the first comparative view of the viral RNA lig-s for RIG-I, MDA5 - LGP2 in the presence of infection.
Palavras-Chave: Human; Immunology; Infectious disease; Microbiology
Imprenta: eLife, v. 5, p. e11275, 2016
Identificador do objeto digital: 10.7554/eLife.11275
Descritores: Chikungunya virus - Genome ; Chikungunya virus - RNA ; Chikungunya Virus - Virus ; Chikungunya virus - Immunology
Data de publicação: 2016
