Chikungunya virus sequences across the first epidemic in Nicaragua, 2014-2015
Autor(es): Wang Chunling, Saborio Saira, Gresh Lionel, Eswarappa Meghana, Wu Diane, Fire Andrew, Parameswaran Poornima, Balmaseda Angel, Harris Eva
Resumo: Chikungunya is caused by the mosquito-borne arthrogenic alphavirus, chikungunya virus (CHIKV). Chikungunya was introduced into the Americas in late 2013 - Nicaragua in mid-2014. Here, we sequenced five imported - 30 autochthonous Nicaraguan CHIKV from cases identified in the first epidemic in the country between August 2014 - April 2015. One full-length - two partial genomic sequences were obtained by deep sequencing; Sanger methodology yielded 33 E1 sequences from five imported - 28 autochthonous cases. Phylogenetic analysis indicates that Nicaraguan CHIKV all belonged to the Asian genotype, Caribbean clade. Moreover, E1 gene sequences revealed accumulation of mutations in later months of the epidemic, including four silent mutations in 11 autochthonous cases - three non-synonymous mutations in three autochthonous cases. No mutations contributing to increased transmissibility by Aedes albopictus were identified in the E1 gene. This represents the most comprehensive set of CHIKV sequences available from the Americas to date.
Imprenta: The American Journal of Tropical Medicine and Hygiene, v. 94, n. 2, p. 400-403, 2016
Identificador do objeto digital: 10.4269/ajtmh.15-0497
Descritores: Chikungunya virus - Cytopathology ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Epidemic ; Chikungunya virus - Public health
Data de publicação: 2016
