Chikungunya virus infections among patients with dengue-like illness at a tertiary care hospital in the Philippines, 2012-2013
Autor(es): Velasco John Mark, Valderama Maria Theresa, Lopez Maria Nila, Chua Domingo, Latog Rene, Roque Vito, Corpuz June, Klungthong Chonticha, Rodpradit Prinyada, Hussem Kittinun, Poolpanichupatam Yongyuth, Macareo Louis, Fernandez Stefan, Yoon In-Kyu
Resumo: Chikungunya virus (CHIKV) often co-circulates with dengue virus (DENV). A cross-sectional surveillance study was conducted at a tertiary hospital in Manila, Philippines, to describe the prevalence - characteristics of DENV - CHIKV infections among patients seeking care for dengue-like illness. Acute blood samples from patients ? 6 months of age clinically diagnosed with dengue from November 2012 to December 2013 underwent reverse transcription polymerase chain reaction (RT-PCR) to detect DENV - CHIKV RNA. A total of 118 patients with clinically diagnosed dengue (age range = 1-89 years, mean = 22 years; male-to-female ratio = 1.51) were tested by DENV RT-PCR; 40 (34%) were DENV PCR-positive (age range = 1-45 years, mean = 17 years). All DENV serotypes were detected: 11 (28%) DENV-1, 6 (15%) DENV-2, 6 (15%) DENV-3, - 17 (42%) DENV-4. Of 112 patients clinically diagnosed with dengue - tested by CHIKV RT-PCR, 11 (10%) were CHIKV PCR-positive (age range = 2-47 years, mean = 20.3 years). No coinfections were detected. Presenting signs/symptoms did not differ between DENV- - CHIKV-positive cases. Sequencing of envelope 1 gene from two CHIKV PCR-positive samples showed Asian genotype. This study highlights the potential for misdiagnosis of medically attended CHIKV infections as DENV infection - the difficulty in clinically differentiating dengue - chikungunya based on presenting signs/symptoms alone. This underscores the necessity for diagnostic laboratory tests to distinguish CHIKV infections in the background of actively co-circulating DENV.
Imprenta: The American Journal of Tropical Medicine and Hygiene, v. 93, n. 6, p. 1318-1324, 2015
Identificador do objeto digital: 10.4269/ajtmh.15-0332
Descritores: Chikungunya virus - RNA ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Dengue ; Chikungunya virus - Epidemiology
Data de publicação: 2015
