Chikungunya virus infection of corneal grafts
Autor(es): Couderc Thérèse, Gangneux Nicolas, Chrétien Fabrice, Caro Valérie, Le Luong Tan, Ducloux Bernadette, Tolou Hugues, Lecuit Marc, Grandadam Marc
Resumo: Chikungunya virus (CHIKV) is an arbovirus with a high potential to spread globally. We investigated whether CHIKV is transmittable via corneal grafts. Serum specimens from 69 potential corneal donors living in La Réunion during the 2005-2006 outbreak of CHIKV infection were screened for anti-CHIKV antibodies. Serum specimens - corneoscleral rims were subjected to quantitative reverse-transcription real-time polymerase chain reaction (qRT-PCR) for detection of CHIKV. CHIKV isolation - immunolabeling were performed on eye tissue specimens. Viral transmission via the ocular route was assessed in an animal model of human CHIKV infection. Twelve apparently uninfected donors were viremic -/or positive for immunoglobulin M (IgM) -/or immunoglobulin G. Eye tissue specimens from 12 donors who were or were not viremic - were or were not seropositive were investigated. qRT-PCR detected CHIKV RNA in corneoscleral rims from 4 patients: 1 patient was viremic, 2 were viremic - IgM positive, - 1 was IgM positive. Infectious CHIKV was isolated from all qRT-PCR-positive samples, - antigens were detected in corneal - scleral specimens, the iris, the ciliary body, - oculomotor muscles. One-third of eligible corneas (4 of 12) from donors apparently uninfected with CHIKV were infected with CHIKV during the study period. CHIKV infects the human cornea - can be transmitted via the ocular route. In the absence of systematic CHIKV screening in donors, cornea donation should be banned in areas where CHIKV circulates.
Imprenta: The Journal of Infectious Diseases, v. 206, n. 6, p. 851-859, 2012
Identificador do objeto digital: 10.1093/infdis/jis296
Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya virus - Antibodies ; Chikungunya virus - Cytokines ; Chikungunya virus - Inflammation ; Chikungunya virus - Viral infections ; Chikungunya virus - PCR detection ; Chikungunya virus - Real Time PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Epidemiology
Data de publicação: 2012
