Chikungunya virus induces a more moderate cytopathic effect in mosquito cells than in mammalian cells
Autor(es): Li Yong-Gang, Siripanyaphinyo Uamporn, Tumkosit Uranan, Noranate Nitchakarn, A-nuegoonpipat Atchareeya, Tao Ran, Kurosu Takeshi, Ikuta Kazuyoshi, Takeda Naokazu, Anantapreecha Surapee
Resumo: Chikungunya virus (CHIKV) is an alphavirus belonging to the Togaviridae family. Alphaviruses cause a chronic non-cytopathic infection in mosquito cells, while they develop a highly cytopathic infection in cells originating from various vertebrates. In this study, we compared the cytopathic effect (CPE) induced by CHIKV in Vero cells - a mosquito cell line, C6/36 cells. CPE - the virus titers were compared between the CHIKV-infected C6/36 - Vero cells. Apoptosis was measured by TUNEL assay, - the differences between the C6/36 - Vero cells were compared. CHIKV infection induced strong CPE - apoptosis in the Vero cells, but light CPE in the C6/36 cells. The virus titers produced in the C6/36 cells were much higher than those produced in the Vero cells. The reason CHIKV induced strong CPE is that this virus triggers strong apoptosis in Vero cells compared with C6/36 cells. CHIKV established a persistent infection in C6/36 cells after being passaged 20 times. CHIKV infection in mosquito cells was distinct from that in Vero cells. The cell - species specificity of CHIKV-induced cell death implies that the cellular - viral regulators involved in apoptosis may play an important role in determining the outcome of CHIKV infection.
Palavras-Chave: Chikungunya virus; Apoptosis; C6/36 cells; Vero cells; Cytopathic effect; Persistent infection
Imprenta: Intervirology, v. 56, n. 1, p. 6-12, 2013
Identificador do objeto digital: 10.1159/000339985
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever
Data de publicação: 2013
