Chikungunya as a cause of acute febrile illness in southern Sri Lanka
Autor(es): Reller Megan E, Akoroda Ufuoma, Nagahawatte Ajith, Devasiri Vasantha, Kodikaarachchi Wasantha, Strouse John J, Chua Robert, Hou Yan'an, Chow Angelia, Sessions October M, Østbye Truls, Gubler Duane J, Woods Christopher W, Bodinayake Champica
Resumo: Chikungunya virus (CHIKV) re-emerged in Sri Lanka in late 2006 after a 40-year hiatus. We sought to identify - characterize acute chikungunya infection (CHIK) in patients presenting with acute undifferentiated febrile illness in unstudied rural - semi-urban southern Sri Lanka in 2007. We enrolled febrile patients ? 2 years of age, collected uniform epidemiologic - clinical data, - obtained serum samples for serology, virus isolation, - real-time reverse-transcriptase PCR (RT-PCR). Serology on paired acute - convalescent samples identified acute chikungunya infection in 3.5% (28/797) patients without acute dengue virus (DENV) infection, 64.3% (18/28) of which were confirmed by viral isolation -/or real-time RT-PCR. No CHIKV/DENV co-infections were detected among 54 patients with confirmed acute DENV. Sequencing of the E1 coding region of six temporally distinct CHIKV isolates (April through October 2007) showed that all isolates posessed the E1-226A residue - were most closely related to Sri Lankan - Indian isolates from the same time period. Except for more frequent - persistent musculoskeletal symptoms, acute chikungunya infections mimicked DENV - other acute febrile illnesses. Only 12/797 (1.5%) patients had serological evidence of past chikungunya infection. Our findings suggest CHIKV is a prominent cause of non-specific acute febrile illness in southern Sri Lanka.
Imprenta: PloS One, v. 8, n. 12, p. e82259, 2013
Identificador do objeto digital: 10.1371/journal.pone.0082259
Descritores: Chikungunya virus - Cytopathology ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Serology ; Chikungunya virus - Viral infections ; Chikungunya virus - Real Time PCR ; Chikungunya virus - RT-PCR ; Chikungunya virus - Serology ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Epidemiology
Data de publicação: 2013
