Characterization of Chikungunya virus infection of a human keratinocyte cell line: Role of mosquito salivary gland protein in suppressing the host immune response
Autor(es): Puiprom Orapim, Morales Vargas Ronald Enrique, Potiwat Rutcharin, Chaichana Panjaporn, Ikuta Kazuyoshi, Ramasoota Pongrama, Okabayashi Tamaki
Resumo: The chikungunya virus (CHIKV) is a mosquito-borne virus that has recently re-emerged in several countries. On infection, the first vertebrate cells to come into contact with CHIKV are skin cells; mosquitoes inoculate the virus together with salivary gl- protein into host skin while probing - feeding on blood. However, there is little known about the susceptibility of human skin cells to CHIKV infection. To clarify this, we investigated the kinetics of CHIKV in the human keratinocyte cell line, HaCaT. CHIKV actively replicated in HaCaT cells, with virus titers in the supernatant increasing to 2.8 × 10(4) plaque-forming units (PFU) ml(-1) 24h post infection. CHIKV infection suppressed production of interleukin-8 (IL-8) in HaCaT cells. The function of IL-8 is to recruit immune cells to virus-infected sites, a process known as chemotaxis. Furthermore, we assessed the role of mosquito salivary gl- protein in CHIKV infections by comparing the levels of CHIKV gene expression - chemokine production in HaCaT cells with - without salivary gl- extract (SGE). SGE enhanced both the expression of the CHIKV gene - the suppression effect of CHIKV on IL-8 production. Our data suggest that the HaCaT cell line represents an effective tool for investigating the mechanism of CHIKV transmission - spread in skin cells. At the mosquito bite site, CHIKV works together with SGE to ensure the virus replicates in skin cells - escapes the host immune system by suppression of IL-8 production.
Palavras-Chave: Chikungunya virus; Human keratinocyte; Salivary gland extract; Chemokine
Imprenta: Infection, Genetics and Evolution, v. 17, p. 210-215, 2013
Identificador do objeto digital: 10.1016/j.meegid.2013.04.005
Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Immune response ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Protein synthesis ; Chikungunya virus - Proteins ; Chikungunya virus - Cytokines ; Chikungunya virus - Immune response ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Immunology
Data de publicação: 2013
