Characterisation of a Chikungunya virus from a German patient returning from Mauritius and development of a serological test
Autor(es): Kowalzik Stefan, Xuan Nghia Vu, Weissbrich Benedikt, Scheiner Barbara, Schied Tanja, Drosten Christian, Müller Andreas, Stich August, Rethwilm Axel, Bodem Jochen
Resumo: We have isolated a Chikungunya (Chik) virus from a patient who returned to Germany after a three-month visit to Mauritius in spring 2006. Upon return she developed a transient fever up to 40 degrees C. This was followed by myalgia - joint pain. IgG antibodies in serum to Chik virus were undetectable. Virus (Chik-Wü1) was isolated on Vero cells. We molecularly cloned the whole genome of Chik-Wü1 from viral RNA by RT-PCR. The complete sequence was determined - functional domains of the genome were assigned. Chik-Wü1 clearly belongs to the group of viruses analysed from the recent Indian Ocean outbreak. In order to develop tools useful for further characterization of Chik-Wü1, we bacterially expressed - purified the capsid (C) - envelope (E) proteins - established an immunoblot assay. Twenty-two of 30 serum samples from Chik virus-infected patients that scored positive in indirect immunofluorescence previously were also reactive in immunoblot analysis with recombinant C - E2 antigens.
Palavras-Chave: Chikungunya virus; Alphavirus; Togavirus; Nucleotide sequence; Recombinant proteins; Immunoblot
Imprenta: Medical Microbiology and Immunology, v. 197, n. 4, p. 381-386, 2008
Identificador do objeto digital: 10.1007/s00430-008-0090-5
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Genome ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya virus - Antibodies ; Chikungunya virus - Viral infections ; Chikungunya virus - RT-PCR ; Chikungunya virus - Serological diagnosis ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever
Data de publicação: 2008
