Broad reactive monoclonal antibodies for rapid identification of enteroviruses show cross-reactivity with Chikungunya virus infected cells
Autor(es): Khairul A H, Chem Y K, Keniscope C, Rosli J, Hassan S, Mat Jais, Chua K B
Resumo: In the past decade, enterovirus 71 (EV71) - chikungunya (CHIK) virus have re-emerged periodically causing serious public health problems in Malaysia, since their first emergence in 1997 - 1998 respectively. This study demonstrates that CHIK virus causes similar patterns of cytopathic effect in cultured Vero cells as some enteroviruses. They also show positive cross-reaction on direct immunofluorescence staining using monoclonal antibodies meant for typing enteroviruses. Without adequate clinical - epidemiological information for correlation, CHIK virus isolated from patients with acute febrile rash can be wrongly reported as untypeable enterovirus due to its cross-reactivity with commercial pan-enterovirus monoclonal antibodies. This is due to the diagnostic laboratory being unaware of such cross-reactions as it has not been reported previously. Final identification of the virus could be determined with specific antibodies or molecular typing using specific oligonucleotide primers for the CHIK virus.
Palavras-Chave: Enterovirus 71; Chikungunya virus; Cytopathic effect; Vero cells; Cross-reaction; Viral diagnosis
Imprenta: The Malaysian Journal of Pathology, v. 32, n. 1, p. 49-52, 2010
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Viral infections ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Molecular screening ; Chikungunya virus - Immunology ; Chikungunya virus - Public health
Data de publicação: 2010
