Autor(es): Hussain Mazhar, Walker Thomas, O'Neill Scott L, Asgari Sassan

Resumo: Aedes aegypti is a blood-feeding mosquito that transmits human pathogens such as Dengue virus, Yellow Fever virus - Chikungunya virus. Recently, dramatic changes in the transcriptome of Ae. aegypti following a blood meal have been reported; however, the molecular factors involved in regulating these changes are largely unknown. In this study, we found induction of a number of endogenous microRNAs (miRNAs) in blood fed (BF) mosquitoes. One of these miRNAs, aae-miR-375, was only detected in BF mosquitoes. Based on target analyses, we found six different genes involved in development - immunity being regulated by aae-miR-375 at the post-transcriptional level. We further confirmed the specific interaction of aae-miR-375 with the target sequences in the transcripts of two immune related genes, cactus - REL1, using a GFP-based reporter assay. Overall, results from this report indicate that miRNAs induced upon blood feeding can regulate the transcript levels of several genes that are important in development - immune responses in mosquitoes. In addition, we demonstrate that aae-miR-375 enhances Dengue virus serotype 2 (DENV-2) infection in an Ae. aegypti cell line.

Palavras-Chave: MicroRNA; Blood meal; Mosquito; aae-miR-375; Gene regulation

Imprenta: Insect Biochemistry and Molecular Biology, v. 43, n. 2, p. 146-152, 2013

Identificador do objeto digital: 10.1016/j.ibmb.2012.11.005

Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Immunology ; Chikungunya virus - Public health

Data de publicação: 2013

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