Assessment of flavaglines as potential Chikungunya virus entry inhibitors
Autor(es): Wintachai Phitchayapak, Thuaud Frédéric, Basmadjian Christine, Roytrakul Sittiruk, Ubol Sukathida, Désaubry Laurent, Smith Duncan R
Resumo: Chikungunya virus (CHIKV) is a re-emerging mosquito-borne alphavirus that recently caused large epidemics in isl-s in, - countries around, the Indian Ocean. There is currently no specific drug for therapeutic treatment or for use as a prophylactic agent against infection - no commercially available vaccine. Prohibitin has been identified as a receptor protein used by chikungunya virus to enter mammalian cells. Recently, synthetic sulfonyl amidines - flavaglines (FLs), a class of naturally occurring plant compounds with potent anti-cancer - cytoprotective - neuroprotective activities, have been shown to interact directly with prohibitin. This study therefore sought to determine whether three prohibitin lig-s (sulfonyl amidine 1 m - the flavaglines FL3 - FL23) were able to inhibit CHIKV infection of mammalian Hek293T/17 cells. All three compounds inhibited infection - reduced virus production when cells were treated before infection but not when added after infection. Pretreatment of cells for only 15 minutes prior to infection followed by washing out of the compound resulted in significant inhibition of entry - virus production. These results suggest that further investigation of prohibitin lig-s as potential Chikungunya virus entry inhibitors is warranted.
Palavras-Chave: Chikungunya virus; Entry inhibitor; Flavaglines; Sulfonyl amidines
Imprenta: Microbiology and Immunology, v. 59, n. 3, p. 129-141, 2015
Identificador do objeto digital: 10.1111/1348-0421.12230
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Protein synthesis ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Vaccine ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Epidemic
Data de publicação: 2015
