Arbovirus vaccines; opportunities for the baculovirus-insect cell expression system
Autor(es): Metz Stefan W, Pijlman Gorben P
Resumo: The baculovirus-insect cell expression system is a well-established technology for the production of heterologous viral (glyco)proteins in cultured cells, applicable for basic scientific research as well as for the development - production of vaccines - diagnostics. Arboviruses form an emerging group of medically important viral pathogens that are transmitted to humans - animals via arthropod vectors, mostly mosquitoes, ticks or midges. Few arboviral vaccines are currently available, but there is a growing need for safe - effective vaccines against some highly pathogenic arboviruses such as Chikungunya, dengue, West Nile, Rift Valley fever - Bluetongue viruses. This comprehensive review discusses the biology - current state of the art in vaccine development for arboviruses belonging to the families Togaviridae, Flaviviridae, Bunyaviridae - Reoviridae - the potential of the baculovirus-insect cell expression system for vaccine antigen production The members of three of these four arbovirus families have enveloped virions - display immunodominant glycoproteins with a complex structure at their surface. Baculovirus expression of viral antigens often leads to correctly folded - processed (glyco)proteins able to induce protective immunity in animal models - humans. As arboviruses occupy a unique position in the virosphere in that they also actively replicate in arthropod cells, the baculovirus-insect cell expression system is well suited to produce arboviral proteins with correct folding - post-translational processing. The opportunities for recombinant baculoviruses to aid in the development of safe - effective subunit - virus-like particle vaccines against arboviral diseases are discussed.
Palavras-Chave: Arbovirus; Subunit vaccine; Virus-like particles; Baculovirus; Insect cells
Imprenta: Journal of Invertebrate Pathology, v. 107, supl, p. S16-S30, 2011
Identificador do objeto digital: 10.1016/j.jip.2011.05.002
Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Vaccine ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Immunology
Data de publicação: 2011
