Arbovirus high fidelity variant loses fitness in mosquitoes and mice
Autor(es): Coffey Lark L, Beeharry Yasnee, Bordería Antonio V, Blanc Hervé, Vignuzzi Marco
Resumo: The error rate of RNA-dependent RNA polymerases (RdRp) affects the mutation frequency in a population of viral RNAs. Using chikungunya virus (CHIKV), we describe a unique arbovirus fidelity variant with a single C483Y amino acid change in the nsP4 RdRp that increases replication fidelity - generates populations with reduced genetic diversity. In mosquitoes, high fidelity CHIKV presents lower infection - dissemination titers than wild type. In newborn mice, high fidelity CHIKV produces truncated viremias - lower organ titers. These results indicate that increased replication fidelity - reduced genetic diversity negatively impact arbovirus fitness in invertebrate - vertebrate hosts.
Imprenta: Proceedings of the National Academy of Sciences of the United States of America, v. 108, n. 38, p. 16038-16043, 2011
Identificador do objeto digital: 10.1073/pnas.1111650108
Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - RNA ; Chikungunya virus - Inflammation ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus
Data de publicação: 2011
