Autor(es)Calzolari Mattia, Bonilauri Paolo, Bellini Romeo, Caimi Marco, Defilippo Francesco, Maioli Giulia, Albieri Alessandro, Medici Anna, Veronesi Rodolfo, Pilani Roberto, Gelati Antonio, Angelini Paola, Parco Valentina, Fabbi Massimo, Barbieri Ilaria, Lelli Davide, Lavazza Antonio, Cordioli Paolo, Dottori Michele

ResumoRecently, Italy-particularly the Emilia-Romagna region-was the location of consecutive outbreaks of human diseases caused by the arboviruses chikungunya virus - West Nile virus. The two outbreaks, spread by different species of mosquitoes, were not related, but pointed out the lack of an arboviral surveillance program in this region. Beginning in 2007 entomological surveillance was initiated in the Emilia-Romagna region, - in 2008 the program was improved - extended at Lombardia region. Using CO(2)-baited traps, 65,292 mosquitoes were collected; pooled by date of collection, location, - species; macerated manually; - tested by reverse transcription (RT)-polymerase chain reaction for the presence of alphaviruses, orthobunyaviruses, - flaviviruses. Amplicons were sequenced - employed for identification of viral RNA by basic local alignment search tool search in GenBank. Results of these assays showed (1) the presence of West Nile virus in two pools of Culex pipiens mosquitoes, (2) the presence of RNA of two orthobunyaviruses, Tahyna virus in a pool of Ochlerotatus caspius mosquitoes - Batai virus in a pool of Anopheles maculipennis mosquitoes, - (3) the presence of flavivirus RNAs in pools of Oc. caspius, Aedes albopictus, - Aedes vexans mosquitoes; the sequences of these amplicons were most closely related to flaviviruses that have been detected only in mosquitoes - had no recognized vertebrate host (Aedes flavivirus, Culex flavivirus, - Kamiti River virus).

ImprentaVector Borne and Zoonotic Diseases, v. 10, n. 9, p. 875-884, 2010

DescritoresChikungunya virus - Arbovirus ; Chikungunya virus - Flaviviridae ; Chikungunya virus - Genome ; Chikungunya virus - Molecular structure ; Chikungunya virus - Pathogenesis ; Chikungunya virus - RNA ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus

Data de Publicação:2010