Antiviral activity of silymarin against Chikungunya virus
Autor(es): Lani Rafidah, Hassandarvish Pouya, Chiam Chun Wei, Moghaddam Ehsan, Chu Justin Jang Hann, Rausalu Kai, Merits Andres, Higgs Stephen, Vanlandingham Dana, Abu Bakar Sazaly, Zandi Keivan
Resumo: The mosquito-borne chikungunya virus (CHIKV) causes chikungunya fever, with clinical presentations such as severe back - small joint pain, - debilitating arthritis associated with crippling pains that persist for weeks - even years. Although there are several studies to evaluate the efficacy of drugs against CHIKV, the treatment for chikungunya fever is mainly symptom-based - no effective licensed vaccine or antiviral are available. Here, we investigated the antiviral activity of three types of flavonoids against CHIKV in vitro replication. Three compounds: silymarin, quercetin - kaempferol were evaluated for their in vitro antiviral activities against CHIKV using a CHIKV replicon cell line - clinical isolate of CHIKV of Central/East African genotype. A cytopathic effect inhibition assay was used to determine their activities on CHIKV viral replication - quantitative reverse transcription PCR was used to calculate virus yield. Antiviral activity of effective compound was further investigated by evaluation of CHIKV protein expression using western blotting for CHIKV nsP1, nsP3, - E2E1 proteins. Briefly, silymarin exhibited significant antiviral activity against CHIKV, reducing both CHIKV replication efficiency - down-regulating production of viral proteins involved in replication. This study may have important consequence for broaden the chance of getting the effective antiviral for CHIKV infection.
Imprenta: Scientific Reports, v. 5, p. 11421, 2015
Identificador do objeto digital: 10.1038/srep11421
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya Virus - Virus ; Chikungunya virus - Vaccine ; Chikungunya virus - Chikungunya fever
Data de publicação: 2015
