An approach towards peptide-based antibody detection for diagnosis of Chikungunya infection
Autor(es): Morey Shweta H, Kashyap Rajpal S, Purohit Hemant J, Taori Girdhar M, Daginawala Hatim F
Resumo: Chikungunya infection, although rarely fatal, is associated with significant morbidity which necessitates its diagnosis in the initial stages. Currently for diagnosis, together with clinical symptoms, immunological methods such as IgG/IgM detection, molecular methods such as real-time reverse transcriptase-polymerase chain reaction - viral isolation methods are available but they are either not very specific or they require high-level sophisticated infrastructures. In the present study, an enzyme-linked immunosorbent assay to evaluate antibody responses to peptides designed from the CHIKV E2 envelope glycoprotein was performed. Synthesized peptides were evaluated with confirmed Chikungunya - non-Chikungunya serum samples for antibody detection. The results demonstrate that the synthetic peptide-based diagnosis of Chikungunya can be an efficient - a more accessible approach in immunodiagnostics.
Palavras-Chave: Chikungunya diagnosis; Peptides; ELISA
Imprenta: Biomarkers, v. 15, n. 6, p. 546-552, 2010
Identificador do objeto digital: 10.3109/1354750X.2010.494200
Descritores: Chikungunya virus - Biochemistry ; Chikungunya virus - Molecular structure ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Viral infections ; Chikungunya virus - Molecular methods ; Chikungunya virus - Real Time PCR ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Molecular screening ; Chikungunya virus - Immunology
Data de publicação: 2010
