Autor(es): Sasao F, Igarashi A, Fukai K

Resumo: Amino acid requirements for the growth of Aedes albopictus, clone C6/36, cells - for the production of dengue (DEN) - Chikungunya (CHIK) viruses were examined by growing the cells or the viruses in media which were deprived of one of the 20 amino acids. Cell growth was markedly inhibited when cystine was omitted from the medium, - to a lesser extent by arginine deprivation. On the other h-, omission of alanine, asparagine, aspartic acid, - glutamic acid at the same time did not affect cell growth. Marked accumulation of alanine was observed in the medium when the cells were grown for 8 days in complete medium, with concomitant depletion of aspartic acid - glutamic acid. The production of CHIK virus was inhibited markedly by omission of cystine from the medium after virus infection, while the production of DEN viruses was more affected by glycine deprivation, although cystine deprivation also inhibited virus production to a lesser extent. On the other h-, production of CHIK - DEN viruses was not affected when alanine, asparagine, aspartic acid, - glutamic acid were omitted from the medium at the same time.

Imprenta: Microbiology and Immunology, v. 24, n. 10, p. 915-924, 1980

Identificador do objeto digital: 10.1111/j.1348-0421.1980.tb02897.x

Descritores: Chikungunya virus - Cell ; Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya Virus - Virus ; Chikungunya virus - Dengue

Data de publicação: 1980

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