Autor(es): Powers A M, Roehrig John T

Resumo: Alphaviruses remain important emerging mosquito-borne, zoonotic pathogens that cause both localized human outbreaks - epizootics (e.g., Venezuelan equine encephalitis) - large human epidemics (e.g., Chikungunya). Alphaviruses are globally dispersed, - each continent has humans at risk from one or more of these arthropod-borne viruses (arboviruses). Symptoms of human alphaviral disease range from frank, severe encephalitis (e.g., eastern - western equine encephalitis) to polyarthritis (e.g., Ross River). Diagnostic techniques to identify human alphaviral infections have changed dramatically with the development - implementation of st-ardized nucleic acid amplification tests (NAAT). The NAAT is rapidly replacing virus isolation - typing using indirect fluorescent antibody (IFA) assay with monoclonal antibodies (MAbs) as the preferred method of virus identification. The older techniques still have value, however, since alphaviral growth in cell culture is rapid, - IFA with MAbs is inexpensive. This chapter provides detailed, st-ardized protocols for the identification of alphaviruses from clinical specimens - the serological characterization of human infection-immune sera. Both laboratory approaches are needed to identify - confirm human infections with these agents.

Palavras-Chave: Alphavirus; Nucleic acid amplification test; ELISA; Monoclonal antibodies

Imprenta: Methods in Molecular Biology, v. 665, p. 17-38, 2011

Identificador do objeto digital: 10.1007/978-1-60761-817-1_2

Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya virus - RT-PCR ; Chikungunya virus - Serological diagnosis ; Chikungunya Virus - Virus ; Chikungunya virus - Epidemic ; Chikungunya virus - Immunology ; Chikungunya virus - Public health

Data de publicação: 2011