A polarized cell model for chikungunya virus infection: entry and egress of virus occurs at the apical domain of polarized cells
Autor(es): Lim Pei Jin, Chu Justin Jang Hann
Resumo: Chikungunya virus (CHIKV) has resulted in several outbreaks in the past six decades. The clinical symptoms of Chikungunya infection include fever, skin rash, arthralgia, - an increasing incidence of encephalitis. The re-emergence of CHIKV with more severe pathogenesis highlights its potential threat on our human health. In this study, polarized HBMEC, polarized Vero C1008 - non-polarized Vero cells grown on cell culture inserts were infected with CHIKV apically or basolaterally. Plaque assays, viral binding assays - immunofluorescence assays demonstrated apical entry - release of CHIKV in polarized HBMEC - Vero C1008. Drug treatment studies were performed to elucidate both host cell - viral factors involved in the sorting - release of CHIKV at the apical domain of polarized cells. Disruption of host cell myosin II, microtubule - microfilament networks did not disrupt the polarized release of CHIKV. However, treatment with tunicamycin resulted in a bi-directional release of CHIKV, suggesting that N-glycans of CHIKV envelope glycoproteins could serve as apical sorting signals.
Imprenta: PLoS Neglected Tropical Diseases, v. 8, n. 2, p. e2661, 2014
Identificador do objeto digital: 10.1371/journal.pntd.0002661
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - RNA ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever
Data de publicação: 2014
