A mouse model of chikungunya virus with utility in antiviral studies
Autor(es): Dagley Ashley, Jul-er Justin G
Resumo: Chikungunya virus (CHIKV) infection generally causes a debilitating arthritis in infected patients. Infection with CHIKV is generally not life-threatening - is associated with a mortality rate <0.1%. However, close to 100% of those infected will develop symptoms of disease, primarily involving swelling - pain of the joints, which can last for months or even years. A model that mimics these symptoms is needed for thedevelopment of therapies to ameliorate disease - control viral infection. In this chapter, we describe the establishment of a model of CHIKV infection in mice that is nonlethal - utilizes footpad swelling - virus titer of various tissues as key disease parameters. This model was developed primarily for use in evaluating the in vivo efficacy of c-idate antiviral agents, although important questions regarding basic biology - pathogenesis of the disease may also be elucidated using this system.
Palavras-Chave: Chikungunya virus; Mouse model; Antiviral; In vivo efficacy
Imprenta: Methods in Molecular Biology, v. 1030, p. 439-448, 2013
Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - Cytopathology ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Cytokines ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus
Data de publicação: 2013
