Insecticide resistance and metabolic mechanisms involved in larval and adult stages of Aedes aegypti insecticide-resistant reference strains from Cuba
Autor(es): Bisset, Juan Andres; Rodriguez, Maria Magdalena; French, Leydis; Severson, David W; Gutierrez, Gladys; Hurtado, Daymi; Fuentes, Ilario
Resumo: Studies were conducted to compare levels of insecticide resistance and to determine the metabolic resistance mechanisms in larval and adult stages of Aedes aegypti from Cuba. Three insecticideresistant reference strains of Ae. aegypti from Cuba were examined. These strains were derived from a Santiago de Cuba strain isolated in 1997; it was previously subjected to a strong selection for resistance to temephos (SAN-F6), deltamethrin (SAN-F12), and propoxur (SAN-F13) and routinely maintained in the laboratory under selection pressure up to the present time, when the study was carried out. In addition, an insecticide-susceptible strain was used for comparison. The insecticide resistance in larvae and adults was determined using standard World Health Organization methodologies. Insecticide resistance mechanisms were determined by biochemical assays. The esterases ( alpha EST and b EST) and mixed function oxidase (MFO) activities were significantly higher in adults than in the larvae of the three resistant strains studied. The association of resistance level with the biochemical mechanism for each insecticide was established for each stage. The observed differences between larval and adult stages of Ae. aegypti in their levels of insecticide resistance and the biochemical mechanisms involved should be included as part of monitoring and surveillance activities in Ae. aegypti vector control programs.
Palavras-Chave: Biological surveys; Insecticides; Insect larvae; Pest control; Hosts; Strains; Resistance mechanisms; Aquatic insects; Public health; Control programs; esterase; Vectors; Expressed sequence tags; Deltamethrin; Aedes aegypti
Imprenta: Journal of the American Mosquito Control Association, v. 30, n. 4, p. 298-304, 2014.
Descritores: Aedes aegypti - Public health
Data de publicação: 2014
