Larvicidal activity of titanium dioxide nanoparticles synthesized using Morinda citrifolia root extract against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus and its other effect on non-target fish
Autor(es): Suman, Thodhal Yoganandham; Ravindranath, Radhika Rajasree Santha; Elumalai, Devan; Kaleena, Patheri Kuny;Ramkumar, Rajendiran; Perumal, Pachiappan; Aranganathan, Lakshminarayanan; Chitrarasu, Paneer Selvam
Resumo: To assess the larvicidal activity of titanium dioxide nanoparticles (TiO sub(2)NP sub(S)) synthesized from the root aqueous extract of Morinda citrifolia (M. citrifolia) against the larvae of Anopheles stephensi (An. stephensi), Aedes aegypti (Ae. aegypti) and Culex quinquefasciatus (Cx. quinquefasciatus). Methods: The M. citrifolia broth solution was prepared by taking 8 g of the powdered root of M. citrifolia in 250 mL Erlenmeyer flask along with 100 mL of distilled water and boiled for 5 min. About 20 mL of M. citrifolia root extract was added into the 80 mL of an aqueous solution of 5 mmol/L TiO(OH) sub(2) for the reduction under continuous stirring for 4 h at 50 degree C. Synthesized TiO sub(2)NP sub(S) were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, field emission scanning electron microscopy and energy dispersive X-ray spectroscopy. X-ray diffraction confirmed the crystalline nature of the nanoparticles. Toxicity studies were carried out against non-target fish species Poecilia reticulata, the most common organism in the habitats of An. stephensi, Ae. aegypti and Cx. quinquefasciatus. Results: The Fourier transform infrared spectroscopy for TiO sub(2)NP sub(S) synthesized by M. citrifolia root extract showed band at 3 426 cm super(-1), 1 637 cm super(-1) and 714 cm super(-1). The 3 426 cm super(-1) showed O-H stretching due to alcoholic group; 1 637 cm super(-1) showed N-H bend due to alcoholic group. In particular, the 1 637 cm super(-1) indicated the presence of H bend bond for 1 degree for proteins. A peak was observed around 714 cm super(-1) due to Ti-O-O bond. Field emission scanning electron microscopy and transmission electron microscopy showed the spherical nature of the nanoparticles with a size of 20.46-39.20 nm. The biosynthesized TiO sub(2)NP sub(S) showed maximum activity against the larvae of An. stephensi, Ae. aegypti and Cx. quinquefasciatus when compared to the aqueous extract of M. citrifolia. Toxicity studies revealed no toxicity towards Poecilia reticulata at LC sub(50) and LC sub(90) doses of TiO sub(2)NP sub(S). Conclusions: TiO sub(2)NP sub(S) could be used along with Poecilia reticulata in integrated vector control.
Palavras-Chave: Infrared spectroscopy; Fourier transforms; Pesticides; Diffraction; Toxicity; Toxicity tests; Roots; Vectors; Habitat; Spectroscopy; Alcoholics; Titanium dioxide; Ionizing radiation; Nanoparticles; Culex quinquefasciatus; Poecilia reticulata; Aedes aegypti; Morinda citrifolia; Anopheles stephensi
Imprenta: Asian Pacific Journal of Tropical Disease, v. 5, n. 3, p. 224-230, 2015.
Descritores: Aedes aegypti - Proteins ; Aedes aegypti - Transmission
Data de publicação: 2015
