Insecticide resistance and genetic variability in natural populations of Aedes (Stegomyia) aegypti (Diptera: Culicidae) from Colombia
Autor(es): Aguirre-Obando, Oscar A.; Dalla Bona, Ana C.; Duque L., Jonny E.; Navarro-Silva, Mário A.
Resumo: Mosquito control prevails as the most efficient method to protect humans from the dengue virus, despite recent efforts to find a vaccine for this disease. We evaluated insecticide resistance and genetic variability in natural populations of Aedes aegypti (Linnaeus, 1762) from Colombia. This is the first Colombian study examining kdr mutations and population structure. Bioassays with larvae of three mosquito populations (Armenia, Calarca and Montenegro) were performed according to the World Health Organization (WHO) guidelines, using Temephos. For the analysis of the Val1016Ile mutation and genetic diversity, we sampled recently-emerged adults from four mosquito populations (Armenia, Calarca, Montenegro and Barcelona). Following the WHO protocol, bioassays implemented with larvae showed resistance to Temephos in mosquito populations from Armenia (77% plus or minus 2) and Calarca (62% plus or minus 14), and an incipient altered susceptibility at Montenegro (88% plus or minus 8). The RR95 of mosquito populations ranged from 3.7 (Montenegro) to 6.0 (Calarca). The Val1016Ile mutation analysis of 107 genotyped samples indicates that 94% of the specimens were homozygous for the wild allele (1016Val) and 6% were heterozygous (Val1016Ile). The 1016Ile allele was not found in Barcelona. Genetic variability analysis found three mitochondrial lineages with low genetic diversity and gene flow. In comparison with haplotypes from the American continent, those from this study suggest connections with Mexican and North American populations. These results confirm that a continuous monitoring and managing program of A. aegypti resistance in the state of Quindio is required.
Palavras-Chave: Population genetics; Bioassays; Insecticides; Genetic isolation; Pest control; Public health; Haplotypes; Gene flow; Mitochondria; Genetic diversity; Vaccines; Mutation
Imprenta: Zoologia, v. 32, n. 1, p. 14-22, 2015.
Descritores: Aedes aegypti - DNA ; Aedes aegypti - Virus ; Aedes aegypti - Vaccine ; Aedes aegypti - Dengue ; Aedes aegypti - Public health
Data de publicação: 2015
