Studies on Bacillus thuringiensis H-14 strains isolated in Egypt. V. Composition and toxicity of the mosquitocidal parasporal inclusions.
Autor(es): Abdel-Hameed, A.; Carlberg, G.; El-Tayeb, O. M.
Resumo: Parasporal inclusions of Bacillus thuringiensis H-14 strains M1 and S128 were characterized by solubilization, electron microscopy, polyacrylamide gel electrophoresis, amino acid analysis and insecticidal activity. Inclusions of both strains are composed largely of protein with 8 to 9% carbohydrate. Amino acid analysis of the purified inclusions revealed that the two strains produce inclusions that are closely related to each other but significantly different from lepidopteran-toxic B. thuringiensis parasporal crystals. The LC sub(50) values of the purified inclusions of strains M1 and S128 were 3.4 and 2.9 ng/ml, respectively, for fourth instar larvae of Aedes aegypti .
Palavras-Chave: Aedes aegypti; Bacillus thuringiensis; Culicidae; Diptera; Amino acid composition; Biological control; Inclusion bodies; Parasporal inclusions; Proteins; Spore crystals; Toxicity
Imprenta: World Journal of Microbiology & Biotechnology, v. 7, n. 2, p. 237-243, 1991.
Descritores: Aedes aegypti - Proteins
Data de publicação: 1991
