Isolation and characterization of densonucleosis virus from Aedes aegypti mosquitoes and its distribution in India
Autor(es): Sivaram, A.; Barde, P. V.; Kumar, S. R. P.; Yadav, P.; Gokhale, M. D.; Basu, A.; Mourya, D. T.
Resumo: Mosquito densonucleosis viruses (DNVs) are known to persistently infect the insect cell line and mosquito population in nature, causing mortality in mosquitoes. Here we report the isolation and characterization of a DNV from Aedes aegypti and its distribution among different Ae. aegypti populations from India. Methods: We screened Ae. aegypti mosquito populations from different states of India by PCR. Virus isolation and purification was performed using a cesium chloride gradient from a positive mosquito colony. Characterization of this isolate was carried out by electron microscopy, Western blot and sequencing. Results: Electron microscopy showed the presence of parvovirus-like particles, and Western blot showed the presence of 2 viral proteins of 40 and 41 kDa. A total of 3,776 bases of genome were sequenced, which included a 3'UTR of 128 bases, a coding region of 3,507 bases and a 5'UTR of 141 bases. Three open reading frames (ORFs) were identified and characterized. The NIVDNV genome showed 95% similarity with Culex pipiens pallens DNV and 93% similarity with Ae. aegypti DNV. Conclusion: Phylogenetic analysis of all 3 ORFs showed that this new isolate falls in the lineage of Brevidensovirus along with other mosquito DNVs. Copyright [copy 2009 S. Karger AG, Basel
Palavras-Chave: Viruses; Densonucleosis virus; Pest control; Mortality causes; Public health; Aedes aegypti; Culex pipiens pallens
Imprenta: Intervirology, v. 52, n. 1, 2009.
Descritores: Aedes aegypti - Cell ; Aedes aegypti - Proteins ; Aedes aegypti - Virus ; Aedes aegypti - Public health
Data de publicação: 2009
