High-throughput PCR assays to monitor Wolbachia infection in the dengue mosquito (Aedes aegypti) and Drosophila simulans
Autor(es): Lee, Siu F.; White, Vanessa L.; Weeks, Andrew R.; Hoffmann, Ary A.; Endersby, Nancy M.
Resumo: We have developed and validated two new fluorescence-based PCR assays to detect the Wolbachia wMel strain in Aedes aegypti and the wRi and wAu strains in Drosophila simulans. The new assays are accurate, informative, and cost-efficient for large-scale Wolbachia screening.
Palavras-Chave: Human diseases; Microbiology; Polymerase chain reaction; Strains; Aquatic insects; Public health; Dengue; Infection
Imprenta: Applied and Environmental Microbiology, v. 78, n. 13, p. 4740-4743, 2012.
Descritores: Aedes aegypti - DNA ; Aedes aegypti - Dengue ; Aedes aegypti - Public health
Data de publicação: 2012