Isolation and characterization of the full coding sequence of a novel densovirus from the mosquito Culex pipiens pallens
Autor(es): Zhai, You-gang; Lv, Xin-jun; Sun, Xiao-hong; Fu, Shi-hong; Gong, Zheng-da; Fen, Yun; Tong, Su-xiang; Wang, Zhao-xiao; Tang, Qing; Attoui, Houssam; Liang, Guo-dong
Resumo: During an investigation of arboviruses in China, a novel densovirus (DNV) was isolated from the adult female Culex pipiens pallens. The virus, designated Culex pipiens pallens densovirus (CppDNV), caused cytopathic effect in C6/36 cells. The virus particles were icosahedral, non-enveloped and had a mean diameter of 24 nm. The complete coding region of CppDNV was found to be 3335 nt and it contained three open reading frames (ORFs). CppDNV shares 82-93 % identical nucleotides with isolates of the Aedes albopictus densovirus [isolates AalDNV-1, AalDNV-2 (C6/36 DNV) and AalDNV- 3], Aedes aegypti densovirus (AaeDNV) and Haemagogus equines densovirus (HeDNV). The nucleotide sequence identity among CppDNV isolates exceeds 98 %. Phylogenetic trees based on non-structural (NS1 and NS2) and capsid (VP) genes show that CppDNV clustered with the species AaeDNV and represents a novel variant of this species within the genus Brevidensovirus.
Palavras-Chave: Viral diseases; Nucleotide sequence; Viruses; Phylogenetics; Capsids; Open reading frames; Haemagogus; Aedes albopictus densovirus; Aedes aegypti densovirus; Culex pipiens pallens; Densovirus
Imprenta: Journal of General Virology, v. 89, n. 1, p. 195-199, 2008.
Descritores: Aedes aegypti - Cell ; Aedes aegypti - Infectious diseases ; Aedes aegypti - Virus ; Aedes aegypti - Public health
Data de publicação: 2008
