Synergisitic Effect of chitinases and Bacillus Thuringiensis israelensis spore-toxin complex against Aedes aegypti larvae
Autor(es): Ramirez-Suero, Montserrat; Valerio-Alfaro, Gerardo; Bernal, Julio S.; Ramirez-Lepe, Mario
Resumo: Six subspecies of Bacillus thuringiensis Berliner (Bt) were grown in minimal medium with chitin as the sole carbon source for 6 days to obtain Bt cell-free fermented broths, which were then evaluated for chitinolytic activity and tested against third-instar Aedes aegypti (L.) (Diptera: Culicidae) larvae. Bt Pakistani showed the highest chitinolytic activity (approximately >2700 mU/mL), Bt kurstaki showed the lowest activity (approximately 2 and approximately 1.4; synergism was not evident for the Serratia marcescens chitinase (synergism factor value approximately 0.9).
Palavras-Chave: Entomologists; Synergism; Chitin; Larvae; Toxicity; Toxicity tests; Mortality causes; Chitinase; Carbon sources; Streptomyces griseus; Bacillus thuringiensis israelensis; Aedes aegypti; Bacillus thuringiensis; Serratia marcescens; Culicidae; Diptera
Imprenta: Canadian Entomologist, v. 143, n. 2, p. 157-164, 2011.
Descritores: Aedes aegypti - Cell
Data de publicação: 2011
